Precast Gel Related Questions

 

 

Gel Type

Bis-Tris

TGN (Tris-Glycine-Novel)

Buffer systems

MOPS and MES

Tris-Glycine (Laemmli)

Features

Clear and sharp bands, high resolution

Quick running, clear bands

Cassette size

Mini Gel
(10 x 8.3 cm)

Midi Gel
(10 X 10 cm)

Mini Gel
(10 x 8.3 cm)

Midi Gel
(10 X 10 cm)

Electrophoresis system

Bio-Rad systems

Mini Gel Tank

Xcell SureLock,

Hoefer SE260

Bio-Rad systems

Mini Gel Tank

XCell SureLock,

Hoefer SE260

Well format &

Capacity

12 wells,

25 μ l/well

15 wells,

22 μ l/well

12 wells,

40 μ l/well

15 wells,

28 μ l/well

12 wells,

25 μ l/well

15 wells,

22 μ l/well

12 wells,

40 μ l/well

15 wells,

 28 μ l/well


Possible Cause: 

Pull the comb out  too slowly and not in a straight direction.

Suggested Solution: 

Pull the comb straightly and quickly out of the cassette to prevent well distortion. Use long loading tip or syringe needle to correct the position of well separators.