Protein Marker Related Questions

 

Yes, 100 uses (5 μL each time) can be expected if freezing and thawing are conducted carefully and properly at the appropriate temperature. Before each use, make sure the protein marker is thoroughly thawed. 

Here are possible causes and solutions

A. May be masked by the prestained protein: extend the electrophoresis time to separate prestained protein from the low molecular weight bands.

B. Not suitable transfer membrane: try to use 0.22 μm PVDF.

C. Inappropriate methanol concentration: 

(a) Use freshly prepared transfer buffer. 

(b) Try to increasing the methanol concentration in the transfer buffer (up to 15% ~ 20% v/v methanol).