Recommendations/Tips for Gel Running
1. Always use fresh 1X running buffer for the inner cathode chamber.
2. Before sample loading, rinse the wells to remove storage buffer.
3. Do not use Tris-Glycine running buffer for GoPAGE™ Bis-Tris Precast Gels.
4. After gel running, keep gel under moisture, and carefully detach the gel from cassette with water.
Sample Preparation for SDS-PAGE
1. Mix protein sample with 2X sample buffer.
2. Heat the diluted samples at 95°C for 5 min or at 70°C for 10 min.
3. Cool the diluted samples to 4°C and spin down the water condensed on tube surface. (If there is high viscosity part at bottom of tube, transfer supernatant to a new tube.)
Prepare GoPAGE™ for Sample Loading
1. Open the plastic bag of GoPAGE™ Precast Gel.
2. Briefly rinse the gel cassette with ddH2O and throw out the gel storage buffer within the wells. Avoid squeezing the gel.
3. Adapt GoPAGE™ to electrophoresis system; instructions are provided below. (BioRad Mini-PROTEAN® Core Electrophoresis System is recommended.)
4. Use a pipette to gently wash the wells with running buffer to remove residual storage buffer.
5. Fill the wells with running buffer prior to sample loading.
6. Load samples and pre-stained protein marker into numbered wells.
7. Fill both inner and outer chambers with running buffer to the highest level. Ensure gel wells are completely covered.
Power Setting for Running GoPAGE™
Optimize the voltage and running time if needed.
Initial (per gel)
Final (per gel)
*1 Running time varies depending on gel percentage, running buffer, temperature, and power supply.
*2 For higher voltage conditions, please use fresh running buffer for inner and outer chambers.
Remove GoPAGE™ Gel from Cassette
Open cassette immediately after electrophoresis. Avoid gel drying.
1. Insert the cassette opener into corners of cassette.
2. Sequentially pry the opener to separate the two plates.
3. Gently pull two plates apart from the top of cassette, allowing gel to rest on one plate. If necessary, use gel remover and water flow to help gel rest on one plate.
4. Carefully detach the gel from the plate with water flow and gel remover.
‒ Under water flow, insert gel remover between gel and cassette from the well site of gel.
‒ Slowly push gel remover to the bottom of gel until gel is fully detached.
‒ Avoid diagonally peeling the gel from the corner.
‒ If necessary, cut well separators with gel remover.
5. Gently remove the gel for further staining or Western blotting.