[PM2700] ExcelBand™ 3-color Broad Range Protein Marker (3.5-245 kDa), 250 μl x 2

145.00 145.0 USD

145.00

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Description 

The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.


Features

  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Two reference bands — 75 kDa (red) and 25 kDa (green)


Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 


Quality Control

Under suggested conditions, PM2700 ExcelBand™ 3-color Broad Range Protein Marker resolves 13 major bands in SDS-PAGE (Bis-Tris gel, MES buffer) and after Western blotting to nitrocellulose membrane. 


Storage

4°C for 3 months
-20°C for 24 months

 

Specification

Cat. No.

PM2700

Series Name

ExcelBand™

Product Size

2 x 250 μl

MW Range

5 – 245 kDa

Band Number

13

Band Color

Red/Green/Blue

Markered Bands

25, 75 kDa

 

Manual

Manual_PM2700_ExcelBand™ 3-color Broad Range Protein Marker

MSDS

MSDS_PM2700

Migration patterns and approximate MWs (kDa)






















 


 

Unraveling the novel effects of aroma from small molecules in preventing hen egg white lysozyme amyloid fibril formation

Zahra Seraj, Arefeh Seyedarabi, Ali Akbar Saboury, Mehran Habibi-Rezaei, Shahin Ahmadian, Atiyeh Ghasemi PLoS One. 2018; 13(1): e0189754. Published online 2018 Jan 22. doi: 10.1371/journal.pone.0189754

PMCID: PMC5777642


The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins

Chih-Ying Lin, Lih-Yuan Lin PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971

PMCID: PMC5790263


Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability

Jia-Shiuan Tsai, Cheng-Han Chao, Lih-Yuan Lin PLoS One. 2016; 11(1): e0147011. Published online 2016 Jan 11. doi: 10.1371/journal.pone.0147011

PMCID: PMC4709241

Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 2 for three columns

Q-PAGE™ Precast Gels

  • User-friendly gel cassette:

    • Numbered and framed wells for sample loading

    • Labeled warning sign and green tape as reminder

  • Enhanced gel performance: 

    • Enhanced gel electrophoresis speed 

    • Better band separation 

    • Stable for shipping at ambient temperature

  • Easy compatibility: 

    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatible with most popular protein electrophoresis systems