[PM5100] ExcelBand™ 3-color Pre-Stained Protein Ladder, High Range (9-245 kDa), 250 μl x 2

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The PM5100 3-color Pre-Stained Protein Ladder High Range is a ready-to-use three-color protein standard with 14 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The PM5100 3-color Pre-Stained Protein Ladder High Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. 


  • Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.

  • Three reference bands 75 kDa (red), 40 kDa (green), and 20 kDa (blue)


Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control 

Under suggested conditions, PM5100 ExcelBand™ 3-color Pre-Stained Protein Ladder High Range resolves 14 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. 


4°C for 3 months
-20°C for 24 months 



Cat. No.


Series Name


Product Size

2 x 250 μl

MW Range

10 – 245 kDa

Band Number


Band Color


Markered Bands

75, 40, 20 kDa



Manual_PM5100_ExcelBand™ 3-color Pre-stained Protein Ladder, High Range 



Migration patterns and approximate MWs (kDa)


Covalent conjugation of extracellular vesicles with peptides and nanobodies for targeted therapeutic delivery

Tin Chanh Pham, 1 , 2 Migara Kavishka Jayasinghe, 1 , 2 , 3 , 4 Thach Tuan Pham, 1 , 2 , 3 , 4 Yuqi Yang, 2 Likun Wei, 2 , 5 Waqas Muhammad Usman, 2 Huan Chen, 2 Marco Pirisinu, 2 Jinhua Gong, 2 , 5 Seongkyeol Kim, 2 Boya Peng, 1 , 3 , 4 Weixi Wang, 2 , 5 Charlene Chan, 6 Victor Ma, 7 Nhung T.H. Nguyen, 8 Dennis Kappei, 6 , 9 Xuan‐Hung Nguyen, 8 William C. Cho, 7 Jiahai Shi,corresponding author 2 , 5 and Minh T.N. Lecorresponding author 1 , 3 , 4,  J Extracell Vesicles. 2021 Feb; 10(4): e12057.

Published online 2021 Feb 16. doi: 10.1002/jev2.12057

PMCID: PMC7886705

Efficient RNA drug delivery using red blood cell extracellular vesicles

Waqas Muhammad Usman, Tin Chanh Pham, Yuk Yan Kwok, Luyen Tien Vu, Victor Ma, Boya Peng, Yuen San Chan, Likun Wei, Siew Mei Chin, Ajijur Azad, Alex Bai-Liang He, Anskar Y. H. Leung, Mengsu Yang, Ng Shyh-Chang, William C. Cho, Jiahai Shi & Minh T. N. Le  

Nat Commun. 2018 Jun 15;9(1):2359. doi: 10.1038/s41467-018-04791-8.

PMCID: PMC6004015 

Unraveling the novel effects of aroma from small molecules in preventing hen egg white lysozyme amyloid fibril formation

Zahra Seraj, Arefeh Seyedarabi, Ali Akbar Saboury, Mehran Habibi-Rezaei, Shahin Ahmadian, Atiyeh Ghasemi

PLoS One. 2018; 13(1): e0189754. Published online 2018 Jan 22. doi: 10.1371/journal.pone.0189754

PMCID: PMC5777642

The conserved basic residues and the charged amino acid residues at the α-helix of the zinc finger motif regulate the nuclear transport activity of triple C2H2 zinc finger proteins

Chih-Ying Lin, Lih-Yuan Lin

PLoS One. 2018; 13(1): e0191971. Published online 2018 Jan 30. doi: 10.1371/journal.pone.0191971

PMCID: PMC5790263

Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability

Jia-Shiuan Tsai, Cheng-Han Chao, Lih-Yuan Lin

PLoS One. 2016; 11(1): e0147011. Published online 2016 Jan 11. doi: 10.1371/journal.pone.0147011

PMCID: PMC4709241

Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference

Odoo - Sample 3 for three columns

YesBlot™ Western Marker I

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)

Odoo - Sample 2 for three columns

Q-PAGE™ Precast Gels

  • User-friendly gel cassette:

    • Numbered and framed wells for sample loading

    • Labeled warning sign and green tape as reminder

  • Enhanced gel performance: 

    • Enhanced gel electrophoresis speed 

    • Better band separation 

    • Stable for shipping at ambient temperature

  • Easy compatibility: 

    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatible with most popular protein electrophoresis systems