RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor that is purified by affinity chromatography from E. coli. This protein inhibits pancreatic-type ribonucleases, RNase A, B, and C by binding strongly to RNases in a noncompetitive mode at a 1:1 ratio. RNAok™ RNase Inhibitor does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV, M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase. Extensive quality control tests ensure RNAok™ RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.
in vitro transcription
20 mM HEPES-KOH (pH 7.6), 50 mM KCl, 8 mM DTT, stabilizer, 50% (v/v) glycerol
-20°C for 24 months