[RP1300] ExcelRT™ Reverse Transcription Kit, 100 Rxn

Call For Price




 

Description 

ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively. 


Features

  • Contains all components for reverse transcription 

  • High yield 

  • Thermostable, up to 50°C, during first strand synthesis 

  • High processivity, generating cDNA up to 8 kb 

  • Reduced RNase H ribonuclease activity 


Application

  • Generation of first strand cDNA from total RNA or mRNA.

  • Suitable for generating cDNA from RNA with strong secondary structure which can be reduced at temperature up to 50°C.


Storage

-20°C for 24 months 


Odoo - Sample 1 for three columns

High yield

 

Odoo - Sample 3 for three columns

High sensitivity


Odoo - Sample 2 for three columns

Thermostable, up to 50°C, during first strand synthesis

 

 

Contents

Component

Volume

ExcelRT™ Reverse Transcriptase (200 U/μl) 

100 μl

RNase Inhibitor (20 U/μl)

100 μl

5X RT Buffer (DTT)

500 μl

dNTP Mix (10 mM each)

200 μl

Oligo (dT)20 (50 μM)

100 μl

Random Hexamers (100 μM)

100 μl

DEPC-Treated H2O

2 x 1 ml


Storage Buffer

Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol

RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol


5X RT buffer (DTT)

250 mM Tris-HCl (pH 8.3), 50 mM DTT, 375 mM KCl 15 mM MgCl2 and 50 mM DTT


Storage

-20°C for 24 months 

 


 

Loss of endogenous estrogen increases cardiac toxicity by doxorubicin

Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong
Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146


Odoo - Sample 1 for three columns

[RP1400] ExcelRT™ Reverse Transcription Kit II

  • Contains all components for reverse transcription

  • High yield 

  • Thermostable, up to 50°C 
    Reduced RNase H ribonuclease activity 

  • Suitable for real-time PCR 

Odoo - Sample 3 for three columns

[TF1000] SMO-HiFi™ DNA Polymerase

  • 5’→3’ DNA polymerase activity

  • 3’→5’ exonuclease (proofreading) activity

  • High reaction rate—10 seconds/kb

  • High fidelity 70 times higher than Taq polymerase

  • Generates blunt end amplicon

  • Thermo-stable for more than 10 hrs at 95°C.

Odoo - Sample 2 for three columns

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High sensitivity and signal intensity 

  • Compatible with fast PCR program

  • With smart blue contrast dye as a visual aid for reaction setup 

  • Low background

  • High stability

Odoo - Sample 3 for three columns

[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR