[TP1000] ExcelTaq™ Taq DNA Polymerase, (5 U/μl, 500 U)

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Description

ExcelTaq™ Taq DNA Polymerase is a recombinant thermo-stable Taq DNA polymerase expressed and purified from an E. coli strain carrying the cloned gene. With a high DNA synthesis rate and high thermo-stability, ExcelTaq™ Taq DNA Polymerase is suitable for common and specialized PCR applications.


Features

  • 5'→3' DNA polymerase activity

  • 5'→3' exonuclease activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3’-dA overhangs

  • Thermo-stable – half-life  lasts for more than 40 min at 95°C


Applications 

  • Routine PCR 

  • Amplification of DNA fragments up to 8 kb

  • Generation of PCR products for TA cloning

  • DNA labeling


Storage

-20°C for 24 months

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Elongation capability

ExcelTaq™ Taq DNA Polymerase can amplify PCR products from λDNA up to 15 kb (M: DM3100).  

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Sensitivity

ExcelTaq™ Taq DNA Polymerase can amplify PCR products from as little as 1 pg of template DNA (M: DM3100).   

 

Contents

Component

Volume

Taq DNA Polymerase (5 U/μl)

100 μl

10X Taq  Buffer

2 x 1 ml


Storage Buffer

20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol


10X Taq buffer

200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% Triton X-100


Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. 


Storage

-20°C for 24 months 

 

Recommended PCR Condition

 

Template

1 – 150 ng

Forward primer

0.1 – 0.5 µM

Reverse primer

0.1 – 0.5 µM

10X Taq Buffer

5 µl

dNTPs

0.2 mM (each)

Taq DNA Polymerase

0.25 µl (1.25U)

H2O

to 50 µl

Total volume

50 µl

 

Recommended PCR Program

Steps

Temp.

Time

Cycles

Template denature

94°C

2 min

1

Denature

94°C

30 sec

25-40

Annealing

50-68°C*

30 sec

Extension

72°C

30 sec/kb

Final extension

72°C

1 min

1

*Optimal PCR condition varies according to primers’ thermodynamic properties.


Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation

Tsan-Jan Chen, Hung-Jung Wang, Jai-Shin Liu, Hsin-Hung Cheng, Sheng-Chieh Hsu, Meng-Chen Wu, Chien-Hung Lu, Yu-Fang Wu, Jing-Wen Wu, Ying-Yuan Liu, Hsing-Jien Kung & Wen-Ching Wang

Commun Biol. 2019; 2: 105. Published online 2019 Mar 15. doi: 10.1038/s42003-019-0343-4

PMCID: PMC6420622



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[RP1000] ExcelRT™ Reverse Transcriptase

  • High yield

  • Thermostable, up to 50°C, during first strand synthesis

  • High processivity, generating cDNA up to 8 kb

  • Reduced RNase H ribonuclease activity

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[TP5000] ExcelTaq™ Hot Start II DNA Polymerase

  • Aptamer-based hot start PCR

  • Reversible enzyme inactivation

  • Omits extra enzyme activation step

  • Convenient for room temperature PCR set-up

  • High yield and specificity of target amplicons

  • Wide range of amplicon length (up to 10 kb)

  • High sensitivity (as low as 1 fg of plasmid)

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[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) 

  • High Stability

  • Fast Hot Start

  • High Sensitivity

  • Low Background / High Specificity 

  • Suitable for Fast Program 

  • Smart Blue Contrast Dye 

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[TP1200] ExcelTaq™ 5X PCR Master Dye Mix

  • 5’→3’ DNA polymerase activity

  • No detectable 3'→5' exonuclease (proofreading) activity

  • Generates PCR products with 3'-dA overhangs

  • High yield PCR 

  • High reproducibility

  • Reduced  pipetting errors

  • Includes tracking dye for direct loading after PCR