[TQ1100] ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX), 200 Rxn

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      Description 

      The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye (Cat. No. CF1000) if recommended by the manufacturer of the qPCR system. 


      Features

      • High sensitivity and signal intensity

      • Smart blue contrast dye as a visual aid for reaction setup

      • Better compatibility for reverse transcription

      • Low background 


      Storage 

      Protected from light.
      Aliquot to avoid multiple freeze-thaw cycles.

      -20°C for 12 months


      Odoo - Sample 1 for three columns

      Sensitivity

      The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.

      Odoo - Sample 2 for three columns

      Better Compatibility for reverse transcription 

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.


      Odoo - Sample 3 for three columns

      Specificity 

      The standard curve (right) and melting curve (left)  of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).



      Contents


      Component

      Volume

      Cat. No

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      2 x 1 ml

      TQ1100 (200 Rxn)

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      5 x 1 ml

      TQ1101 (500 Rxn)


      Storage 

      Protected from light. 
      Aliquot to avoid multiple freeze-thaw cycles.

      -20°C for 12 months

       


       

      Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.

      Satnam Singh  Suneet Pandher  Mridula Gupta  Gurmeet Kaur  Pankaj Rathore

      Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328


      Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells 

      Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056

      PMCID: PMC5522218


      Hydroxylation and sulfation of sex steroid hormones in inflammatory liver 

      Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031

      PMCID: PMC5706436


      Loss of endogenous estrogen increases cardiac toxicity by doxorubicin

      Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong

      Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146




      ExcelTaq™ 2X Q-PCR Master Mix

      Product Name

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

      ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

      ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

      ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

      ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

      Cat. No.

      TQ1100

      TQ1110

      TQ1200

      TQ1210

      TQ2110

      Detection chemistry

      SYBR

      SYBR

      SYBR

      SYBR

      TaqMan

      Blue contrast dye for best convenience

      ü

      ü

      ü

      ü

      X

      ROX dye for reference

      X

      ü

      X

      ü

      ü

      Time required for enzyme activation

      10 min

      10 min

      2 min

      2 min

      10 min

      qPCR program

      Standard

      Standard

      Fast and Standard

      Fast and Standard

      Standard