[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 Rxn

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Description 

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. 

With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.


Features

  • High Stability

  • Fast Hot Start

  • High Sensitivity

  • Low Background / High Specificity 

  • Suitable for Fast Program 

  • Smart Blue Contrast Dye 


Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles. 

-20°C for 12 months

Odoo - Sample 1 for three columns

Fast Hot Start

The overlapped amplification curves from different hot start duration display fast activation of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX). 

The initial activation step was set as 2,5, and 10 minutes. TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) contains a special hot-start Taq polymerase that omits time-consuming initial activation step required by chemically modified hot start Taq polymerases (Brand A). 


Odoo - Sample 2 for three columns

High Sensitivity

The amplification curves from different amounts of cDNA display high sensitivity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX). 

The sensitivity assay of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) was examined with serial diluted cDNA from 25 ng to 1.4 fg as templates. The equal intervals of amplification curves also displayed the high precision of TQ1200 in target quantification.


Odoo - Sample 3 for three columns

Low Background

The single peak of melting curve displays high specificity of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX).

The post-amplification melting-curve analysis was examined with 5 ng cDNA as template, abnormally high concentration of primers (400 nM), and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) or qPCR master mix from brand A as reagents. 

Odoo - Sample 1 for three columns

High Stability

The overlapped amplification curves and melting curves from different storage conditions display high stability of TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX). 

Stability was analyzed via real-time PCR amplification with 1 ng cDNA as template, and TQ1200 ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) stored at -20°C, 4°C, 22°C and 37°C for 30 days as reagents.


Odoo - Sample 2 for three columns

Suitable for Fast Program


To add a fourth column, reduce the size of these three columns using the right icon of each block. Then, duplicate one of the column to create a new one as a copy.

Odoo - Sample 3 for three columns

Smart Blue Contrast Dye

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Contents


Component

Volume

Cat. No

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

2 x 1 ml

TQ1200 (200 Rxn)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

5 x 1 ml

TQ1201 (500 Rxn)


Storage 

Protected from light. 
Aliquot to avoid multiple freeze-thaw cycles.

-20°C for 12 months

 


 

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ExcelTaq™ 2X Q-PCR Master Mix

Product Name

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX)

ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX)

ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)

Cat. No.

TQ1100

TQ1110

TQ1200

TQ1210

TQ2110

Detection chemistry

SYBR

SYBR

SYBR

SYBR

TaqMan

Blue contrast dye for best convenience

ü

ü

ü

ü

X

ROX dye for reference

X

ü

X

ü

ü

Time required for enzyme activation

10 min

10 min

2 min

2 min

10 min

qPCR program

Standard

Standard

Fast and Standard

Fast and Standard

Standard