[WM1000] YesBlot™ Western Marker I, 250 μl

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Description 

YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgG-binding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading. 


Features

  • Ready-to-use — no need of mixing or heating before sample loading

  • Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots

  • Pre-stained bands — 4 prestained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Wide range — 10 clear bands from 15 to 200 kDa for size estimation

  • Quick reference — two enhanced bands (30 and 80 kDa)


Contents 

The YesBlot™ Western Marker I contains recombinant IgG binding proteins, Glycerol, SDS, and tracking dyes in a Tris-HCl buffer. 


Storage

4°C for 3 months
-20°C for 24 months

 

Specification

Cat. No.

WM1000

Series Name

YesBlot™

Product Size

250 μl

MW Range (Western Bands)

15 – 200 kDa

MW Range (Pre-stained Bands)

10 – 70 kDa

Band Number (Western Bands)

10 

Band Number (Pre-stained Bands)

Pre-stained Band Color

Pink/Green/Blue


Technical information

Principle of YesBlot™ Western Marker I

 
 













 

Manual

Manual_WM1000_YesBlot™ Western Marker I

MSDS

MSDS_WM1000


Flyer

Western Marker- Powerful for Western Data Credibility


Migration patterns and approximate MWs (kDa) of pre-stained bands






 


Odoo - Sample 1 for three columns

ExcelBand™ Protein Markers

  • Ready-to-use— premixed with a loading buffer for direct loading, no need to boil

  • Broad range310 kDa to 5 kDa

  • Pre-stained bands for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane

  • Enhanced bands— for quick reference


Odoo - Sample 2 for three columns

Q-PAGE™ Precast Gels

  • User-friendly gel cassette:

    • Numbered and framed wells for sample loading

    • Labeled warning sign and green tape as reminder

  • Enhanced gel performance: 

    • Enhanced gel electrophoresis speed 

    • Better band separation 

    • Stable for shipping at ambient temperature

  • Easy compatibility: 

    • Available as homogeneous and adjusted gradient gels for a wide range of protein separation.

    • Compatible with most popular protein electrophoresis systems